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1 School of Food Technology, The University of New South Wales, P.O. Box 1,
Kensington, NSW 2033, Australia
2 Waters Associate Pty. Ltd., P.O. Box 145, Chippendale, NSW
2008 Australia.
Estimation of total soluble protein in must and wine by high-performance liquid chromatography is achieved with a size exclusion column. A protein standard, bovine serum albumin, is eluted from this column with linear response to a concentration of 1 g/L. Protein is separated from other UV absorbing components of must and wine and estimated at 280 nm on a Waters Protein Separation System with a 0.1-M ammonium acetate mobile phase containing 10% glycerol. The method is now used routinely for estimation of protein in must and wine.
Submitted on March 14, 1980
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