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Am. J. Enol. Vitic. 32:3:241-243 (1981)
Copyright © 1981 by the American Society for Enology and Viticulture.
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Estimation of Soluble Protein in Must and Wine by High-Performance Liquid Chromatography

Paul J. Tyson 1, Ernesto S. Luis 1, W. Roy Day 2, Brian Walker 2, and Terence H. Lee 1

1 School of Food Technology, The University of New South Wales, P.O. Box 1, Kensington, NSW 2033, Australia
2 Waters Associate Pty. Ltd., P.O. Box 145, Chippendale, NSW 2008 Australia.

Estimation of total soluble protein in must and wine by high-performance liquid chromatography is achieved with a size exclusion column. A protein standard, bovine serum albumin, is eluted from this column with linear response to a concentration of 1 g/L. Protein is separated from other UV absorbing components of must and wine and estimated at 280 nm on a Waters Protein Separation System with a 0.1-M ammonium acetate mobile phase containing 10% glycerol. The method is now used routinely for estimation of protein in must and wine.

Submitted on March 14, 1980
Revised on March 23, 1981
Accepted on April 8, 1981







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Copyright © 1981 by the American Society for Enology and Viticulture.