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Am. J. Enol. Vitic. 40:4:283-291 (1989)
Copyright © 1989 by the American Society for Enology and Viticulture.
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Analysis of Anthocyanins by High Performance Liquid Chromatography-Diode Array Spectroscopy in a Hybrid Grape Variety (Vitis vinifera x Vitis berlandieri 41B)

E. Hebrero 1, C. Garcia-Rodriguez 1, C. Santos-Buelga 1, and J. C. Rivas-Gonzalo 1

1 Departamento de Quimica Analitica, Nutricion y Bromatologia, Facultad de Farmacia, Universidad de Salamanca. 37007 Salamanca, Spain.

The mono- and diglucoside anthocyanins of a variety of hybrid grapes were separated, identified, and quantified by joint use of high performance liquid chromatography with diode array spectroscopic detection. The order of elution and the spectral properties observed permitted correct identification of 20 compounds. The presence of a shoulder at 440 nm in the visible-ultraviolet spectrum of the monoglucoside derivatives showed that they are different from the diglucosides. Moreover, study of the spectrum in the uv region pointed to the existence of anthocyanins acylated with p-coumaric acid. Identification was confirmed by analysis of the chromatograms recorded at different wavelengths and after performing acid and alkaline hydrolyses.

Key words: anthocyanins, HPLC

Submitted on November 15, 1988




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[Abstract] [Full Text] [PDF]


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Copyright © 1989 by the American Society for Enology and Viticulture.