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1 Department of Food Science and Technology,
Cornell University, New York State Agricultural Experiment Station, Geneva, NY 14456
The malolactic fermentation has been shown to be an energy-yielding process in six strains of lactic acid bacteria which contain the full malolactic enzyme system. The mechanism for this reaction is chemiosmotic. A mutant strain with a defective malolactic enzyme did not produce ATP during malate catabolism; the parent strain did produce ATP during malate catabolism. A strain of Lactobacillus plantarum that is naturally malolactic-deficient does not produce ATP when given L-malate as a substrate. The data support the hypothesis that the malolactic enzyme system has evolved as an energy-producing mechanism for lactic acid bacteria adapted to the low pH of wine.
Key words: enzyme, lactic acid bacteria, malate, malolactic fermentation
Submitted on July 31, 1989
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