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Am. J. Enol. Vitic. 44:2:211-216 (1993)
Copyright © 1993 by the American Society for Enology and Viticulture.
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The Effect of Elemental Sulfur, Yeast Strain, and Fermentation Medium on Hydrogen Sulfide Production During Fermentation

Carla S. Thomas 1, Roger B. Boulton 2, Michael W. Silacci 3, and W. Douglas Gubler 1

1 Department of Plant Pathology, University of California, Davis, CA 95616
2 Department of Viticulture and Enology, University of California, Davis, CA 95616
3 Viticulturist, Beaulieu Vineyard, Rutherford, CA, 94573.

The effect of elemental sulfur on hydrogen sulfide production was investigated in stirred fermentations of a reconstituted commercial white grape juice or a defined juice-like medium. Nine commercial wine yeast strains and three levels of elemental sulfur were studied (0, 1.7, and 3.4 mg/L fermentation) in 117 trials. These levels represent the observed elemental sulfur residues at harvest in commercial vineyards for the 1989 and 1990 seasons (12). The cumulative hydrogen sulfide produced was measured using cadmium hydroxide traps that were collected at 24-hour intervals for five days. Two peaks of hydrogen sulfide production occurred during the fermentations. Peak 1 occurred earlier and was more intense than Peak 2. The two peaks occurred independently of each other. Peak 1 was associated with the yeast strain that was used. Peak 2 was associated with the medium that was used. The amount of elemental sulfur added to the medium was not related to the amount of hydrogen sulfide produced in these fermentations. Specific yeast-medium-sulfur interactions were apparent.

Key words: elemental sulfur, hydrogen sulfide, fermentation, wine, Saccharomyces cerevisiae

Submitted on January 22, 1992




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A. L. Linderholm, C. L. Findleton, G. Kumar, Y. Hong, and L. F. Bisson
Identification of Genes Affecting Hydrogen Sulfide Formation in Saccharomyces cerevisiae
Appl. Envir. Microbiol., March 1, 2008; 74(5): 1418 - 1427.
[Abstract] [Full Text] [PDF]




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Copyright © 1993 by the American Society for Enology and Viticulture.