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Am. J. Enol. Vitic. 44:3:249-254 (1993)
Copyright © 1993 by the American Society for Enology and Viticulture.
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Tumor Formation by Agrobacterium tumefaciens Is Suppressed by Agrobacterium radiobacter HLB-2 on Grape Plants

XIN-AN PU 1 and R. N. GOODMAN 1

1 Department of Plant Pathology, 108 Waters Hall, University of Missouri, Columbia, MO 65211.

Agrobacterium radiobacter HLB-2 inhibits Agrobacterium tumefaciens in vitro and tumor development on grape stem explants. The suppression of tumor formation was more effective when HLB-2 was inoculated either prior to a virulent strain or simultaneously, and at a higher cell concentration. Grape explants were transformed 0 to 48 hours after wounding (conditioning). Both A. radiobacter strains HLB-2 and K84, and A. tumefaciens strains are able to multiply similarly in grape explants. The ability of HLB-2 to suppress tumors does not appear to be merely a result of competition for nutrients.

Key words: Agrobacterium, tumor formation, bacterial antagonist, agrocin

Submitted on July 6, 1992






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Copyright © 1993 by the American Society for Enology and Viticulture.