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1 Nietvoorbij Institute
for Viticulture and Oenology, Private Bag X5026, Stellenbosch 7599, Republic of South Africa
2 Department of Medical Biochemistry, University of Stellenbosch,
P.O. Box 19063, Tygerberg 7505, Republic of South Africa.
An inexpensive and high yielding procedure is described for the isolation of DNA from small quantities of leaves or roots from Vitis vinifera. The buffer capacity of the extraction solution was found to be the single most important factor in the isolation of the DNA. Different detergent (CTAB) concentrations have an effect on the efficiency of cleavage by restriction endonucleases. The quality of the DNA was checked by fingerprint analysis after digestion with HaeIII and Hinfl restriction enzymes and probing with the multilocus pV47-2 probe. Differences between Vitis vinifera cultivars were detected.
Key words: Vitis, grapevines, DNA, fingerprinting
Submitted on October 5, 1992
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