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Am. J. Enol. Vitic. 45:4:460-463 (1994)
Copyright © 1994 by the American Society for Enology and Viticulture.
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Determination of Biogenic Amines and Other Amines in Wine by an Optimized HPLC Method With Polarity Gradient Elution

M. Iñiguez Crespo 1 and B. Vázquez Lasa 1

1 Estación Enológica de Haro. C/ Bretón de los Herreros, 4, 26220 Haro (La Rioja), Spain.

An optimized method to determine biogenic amines and other amines in wine with pre-column derivatization with o-phthaldialdehyde is reported. Determination of the different amines has been carried out using a polarity gradient elution technique involving methanol and sodium acetate buffer. The method has been optimized in terms of its precision and accuracy assessed by statistical means using formula derived for the purpose of treating determined number sets of data. After validation, the method was applied to determine amines in red, white, and rosé wines of Rioja region.

Key words: amines, wine, optimization polarity gradient

Submitted on October 22, 1993






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Copyright © 1994 by the American Society for Enology and Viticulture.