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Am. J. Enol. Vitic. 46:1:43-48 (1995)
Copyright © 1995 by the American Society for Enology and Viticulture.
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Plasmid and Chromosome Characterization of Leuconostoc oenos Strains

H. Priévost 1, J. F. Cavin 1, M. Lamoureux 1, and C. Diviès 1

1 Laboratoire de Microbiologie, ENSBANA (Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation), 1, esplanade Erasme. Université de Bourgogne 21000 Dijon, France.

Fifteen strains of Leuconostoc oenos were examined for plasmid content. Six were found to contain single cryptic plasmids designated pUBL01 to pUBL06 and ranging in size from 3.9 to 26 kb. Through size analysis, restriction endonuclease mapping, and DNA-DNA hybridization, the group of six plasmids comprised three plasmids which were not homologous. Pulsed field electrophoresis and restriction fragment length polymorphism mapping were used to differentiate strains harboring plasmid DNA. The genome size ranged from 1770 to 1975 kb. EcoRI and Hind III RFLP were suitable to differentiate the strains in the region of 9- to 23-kb fragments. Two strains with identical RFLP contained different plasmids useful for their recognition.

Key words: Leuconostoc oenos, plasmid, restriction profile, pulsed field gel electrophoresis, chromosome sizing

Submitted on August 5, 1993






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Copyright © 1995 by the American Society for Enology and Viticulture.