Comparison by Isozyme and RAPD Analysis of Some Isolates of the Grapevine Dieback Fungus, Eutypa lata

¹ Institut National de la Recherche Agronomique, Station de Recherches Viticoles, 34751 Villeneuve-les-Maguelone Cedex, France
² Institut National de la Recherche Agronomique, Station Amélioration des Plantes, 2, Place Viala, 34060 Montpellier Cedex 1, France.

The purpose of this study was to identify molecular markers suitable for developing studies on the genetic variation and biology of the grapevine dieback fungus, Eutypa lata. Using a preliminary set of 18 isolates, 21 isozyme markers were identified in nine isozyme systems and 18 random amplified polymorphism DNA (RAPD) markers were detected with six different 10-mer primers. A high level of genetic variation was found among isolates originating from different geographic locations. Polymorphism was also detected among isolates from the same stroma and the different stromas were clearly separated in the cluster analysis based on RAPD data. No association was found between the molecular patterns and either the host or the geographic proximity of the isolates. Isolates having a low pathogenicity on grapevine appeared genetically similar to other isolates. The isolate AM14 was clearly distinguished by both types of markers, and its taxonomic position is discussed. RAPDs appeared more promising than isozymes for the study of genetic variation of E. lata and for fingerprinting isolates with specific pathogenic traits.

Key words: Eutypa lata, grapevine dieback

Submitted on March 1, 1995

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