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1 Instituto Nacional de Investigação Agrária, 2560 Dois Portos,
Portugal
2 Instituto Superior de Agronomia, Universidade Técnica de Lisboa, 1399 Lisboa
Codex, Portugal.
The isoenzymatic activity of 
-esterase (EC 3.1.1.1), acid phosphatase (EC 3.1.3.2), glucosephosphate
isomerase (EC 5.3.1.9) and phosphoglucomutase (EC 2.7.5.1) was studied in young leaves, obtained from
cuttings developed in a room with controlled environment. The zymograms of 29 grapevine cultivars grown in
Portugal and some of their synonyms were analyzed, in order to authenticate synonymous names. The data
analysis was performed by a numerical taxonomic method. We detected 24 polymorphic bands, which
enabled the differentiation of 29 grapevine cultivars, using the four isoenzymatic systems. The principal area
of application of this methodology is the resolution and confirmation of difficult cases of synonyms or
homonyms in ampelography. In this study, we confirmed that Pedernã from Vinhos Verdes is a synonym for
Arinto from Bucelas; Maria Gomes from Bairrada for Fernão Pires from Ribatejo; Periquita from Setúbal for
João de Santarém from Oeste and for Trincadeira from Bairrada; Trincadeira Preta from Ribatejo for Crato
Preto from the Algarve and for Tinta Amarela from Douro. We also concluded that Trincadeira Preta from
Ribatejo is different from the homonymous Trincadeira from Bairrada.
Key words: ampelography, isoenzyme, leaf, Vitis, chemotaxonomy
Submitted on May 2, 1996
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