AJEV
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Enol. Vitic. 49:4:403-407 (1998)
Copyright © 1998 by the American Society for Enology and Viticulture.
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lin, H.
Right arrow Articles by Walker, M. A.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Lin, H.
Right arrow Articles by Walker, M. A.
Agricola
Right arrow Articles by Lin, H.
Right arrow Articles by Walker, M. A.

Identifying Grape Rootstocks With Simple Sequence Repeat (SSR) DNA Markers

Hong Lin 1 and M. Andrew Walker 1

1 Department of Viticulture and Enology, University of California, Davis, CA 95616-8749.

awalker{at}ucdavis.edu

Identification of grape rootstocks using morphological features can be difficult because foliar characteristics are variable and often hard to distinguish. This dependence on morphological traits makes rootstock identification nearly impossible during the dormant season, with properly disbudded rootstock plantings, or any time shoots from below the graft union are infrequent. However, cambium tissue from under the rootstock bark provides an alternative sample material for DNA analysis when actively growing shoot tips are not available. DNA was extracted from the leaves and cambium of 58 rootstock selections from the University of California, Davis collections and tested with seven simple sequence repeat (SSR) primers (VVMD5, VVMD6, VVMD7, VVMD28, VVMD31, VVS2, and VVS29). The use of VVMD31, VVS2, VVMD7, and VVMD5 unambiguously identified all of the rootstocks. This technique provides an excellent means of rootstock identification and new variety "fingerprinting".

Key words: fingerprinting, microsatellite, rootstock identification, ampelography, Vitis species

Submitted on February 18, 1998




This article has been cited by other articles:


Home page
 Am. J. Enol. Vitic.Home page
M. T. de Andres, J. A. Cabezas, M. T. Cervera, J. Borrego, J. M. Martinez-Zapater, and N. Jouve
Molecular Characterization of Grapevine Rootstocks Maintained in Germplasm Collections
Am. J. Enol. Vitic., March 1, 2007; 58(1): 75 - 86.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the American Society for Enology and Viticulture.