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Am. J. Enol. Vitic. 54:1:56-58 (2003)
Copyright © 2003 by the American Society for Enology and Viticulture.
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Verifying the Identity and Parentage of Cruzado de Rabo de Ovelha with Microsatellite Markers

Ricardo Magalhaes 1, Miguel A. Faria 2, Nuno M.M. Santos 3, J.E. Eiras Dias 3, Nuno Magalhaes 4, Carole P. Meredith 5, and F Ferreira Monteiro 6

1 ICBAS, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Porto, PortugalIPVE, Instituto Português de Viticultura e Enologia, Maia, Portugal
2 ICBAS, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Porto, Portugal
3 Estação Vitivinícola Nacional, Dois Portos, Portugal
4 Universidade de Trás-os-Montes e Alto Douro, Vila Real, Portugal
5 Department of Viticulture and Enology, University of California, Davis, CA.
6 IPVE, Instituto Português de Viticultura e Enologia, Maia, Portugal

email: ffmont@ mail.telepac.pt

The reported parentage of the grape variety Cruzado de Rabo de Ovelha was examined by the use of 12 microsatellite DNA markers. The microsatellite profile of Cruzado de Rabo de Ovelha was identical to that of Castelo Branco, supporting ampelographic analysis indicating they are the same variety. Data are consistent with the parentage of Castelo Branco being Fernão Pires and Diagalves as reported. Rabo de Ovelha was ruled out as a parent of Cruzado de Rabo de Ovelha at 4 of the 12 loci.

Note:
Acknowledgment: Markers for the 12 microsatellite loci provided by C.P. Meredith.

Key words: SSR, genetics, molecular markers, Portugal






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