Survey of Five Indigenous Istrian Cultivars for the Presence of Six Grape Viruses

Danijela Poljuha ¹, Barbara Sladonja ¹, and Ðordano Peruriæ ¹

¹ Department of Agriculture and Food Science, Institute of Agriculture and Tourism, pp. 31, 52440 Poreè, Croatia

email: danijela{at}iptpo.hr

Five commercially important indigenous grapevine cultivars (Vitis vinifera L.) were surveyed for the presence of virus and virus-like diseases during collection, clonal selection, and grapevine genotyping in Istria, Croatia. Six viruses were tested by ELISA: grapevine fanleaf virus (GFLV), grapevine fleck virus (GFkV), Arabis mosaic virus (ArMV), grapevine virus A (GVA), and grapevine leafroll-associated virus 1 and 3 (GLRaV-1 and GLRaV-3). All viruses except ArMV were detected and one cultivar was 100% infected by GLRaV-3. Mixed infections, most frequently by GFkV and GLRaV-3, were also identified.

Note:
Acknowledgments: This work was supported by funds from the Croatian Ministry of Science and Technology and Local Authorities of Matulji. The authors thank Irma Toma[unknown]iè for valuable assistance.

Key words: grape viruses, Vitis vinifera L., indigenous varieties, ELISA test