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Development of Grape Microsatellite Markers and Microsatellite Analysis Including Oriental Cultivars

¹ National Research Institute of Brewing, 3-7-1, Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan; ² Shimane Winery, Co., Ltd., 264-2, Hishine, Taisha-cho, Shimane 699-0733, Japan; ³ Hokkaido Sugar Co., Ltd., 3-753-2, Shinko-chuo, Ishikari, Hokkaido 061-3242, Japan; and ⁴ School of Biological Sciences, University of Bristol, Woodland Road, Bristol, BS8 1UG, UK.

^* Corresponding author [fax: +81-82-420-0803; email: gotoh_n{at}nrib.go.jp]

Nine newly developed microsatellite markers for grapes were characterized. Using these markers and eight reported markers, seven Occidental and eight Oriental cultivars were analyzed, including Japanese and Chinese cultivars of Vitis vinifera, two cultivars of V. labrusca, and one sample each of V. riparia and V. rotundifolia. Calculated phenetic distances (1 – proportion of shared alleles) agreed well with the classification of grapes. A dendrogram based on the phenetic distances showed a clear separation of species of Vitis, as well as Oriental and Occidental cultivars. Results suggested the importance of Oriental cultivars as genetic resources of grapes.

Key words: microsatellite DNA, SSR, grape, Oriental cultivar