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Am. J. Enol. Vitic. 57:4:519-525 (2006)
Copyright © 2006 by the American Society for Enology and Viticulture.
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Research Note

Identification of Lactic Acid Bacteria from Vinegar Flies Based on Phenotypic and Genotypic Characteristics

Willem H. Groenewald1, Carol A. Van Reenen1, Svetoslav D. Todorov1, Maret Du Toit2, R. Corli Witthuhn3, Wilhelm H. Holzapfel4 and Leon M.T. Dicks1,*

1 Department of Microbiology, 2 Institute for Wine Biotechnology, and 3 Department of Food Science, University of Stellenbosch, 7600 Stellenbosch, South Africa; and 4 Federal Research Centre for Nutrition, Institute of Hygiene and Toxicology, Haid-und-Neu-Strasse 9, D-76131 Karlsruhe, Germany.

* Corresponding author [fax: 27 21-8085846; email: lmtd{at}sun.ac.za]

Thirty lactic acid bacteria were isolated from the intestinal tract of Drosophila simulans Stuvervant collected from a winery in the Stellenbosch region, South Africa. The isolates were grouped according to morphological, biochemical, and physiological characteristics. Strains selected from each group were identified to species level by PCR with species-specific primers, PCR-based DGGE, and 16S rDNA sequencing. The majority of isolates belonged to the species Lactobacillus plantarum, but Lactobacillus paracasei, Lactobacillus sanfranciscensis, Leuconostoc mesenteroides subsp. mesenteroides, Lactococcus lactis subsp. lactis, Enterococcus faecalis and Pediococcus pentosaceus were also identified. The genotypic relatedness between strains of L. plantarum was determined by RAPD-PCR.

Key words: lactic acid bacteria, vinegar flies, Drosophila






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