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Stilbene-Synthase Gene Expression after Aspergillus carbonarius Infection in Grapes

¹ Istituto di Frutti-Viticoltura and ² Istituto di Entomologia e Patologia Vegetale, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, I-29100 Piacenza, Italy.

^* Corresponding author (email: luigi.bavaresco{at}unicatt.it; fax: +39 0523 599268)

Berries of Vitis vinifera L. cv. Barbera (disease susceptible) and the interspecific variety Castor (disease resistant) were infected at veraison by the ochratoxin A-producing fungus Aspergillus carbonarius in order to test the stilbene-synthase gene expression by reverse transcriptase-polymerase chain reaction. Stilbene (trans-resveratrol and piceatannol) synthesis was also analyzed. Stilbene-synthase gene expression was induced by the fungus and stilbenes were produced in both varieties, but was significantly higher in Castor. Castor displayed few disease symptoms while Barbera was highly infected.

Key words: grapevine, stilbene synthase, Aspergillus carbonarius, trans-resveratrol