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Am. J. Enol. Vitic. 58:1:75-86 (2007)
Copyright © 2007 by the American Society for Enology and Viticulture.
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Molecular Characterization of Grapevine Rootstocks Maintained in Germplasm Collections

María Teresa de Andrés1,*, José Antonio Cabezas2, María Teresa Cervera3, Joaquín Borrego1, José Miguel Martínez-Zapater2 and Nicolás Jouve4

1 Departamento de Agroalimentación, Instituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentario, Finca El Encín, Alcalá de Henares, Madrid, Spain; 2 Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología-CSIC, Cantoblanco, 28049 Madrid, Spain; 3 Departamento de Sistemas y Recursos Forestales, CIFOR, Instituto Nacional de Investigación Agraria y Alimentaria, Carretera de A Coruña, 28049 Madrid, Spain; and 4 Departamento de Biología Celular y Genética, Campus de la Universidad de Alcalá de Henares, 28771 Madrid, Spain.

* Corresponding author (email: maite.deandres{at}madrid.org)

Grapevine rootstocks are a complex group of plants, most of them hybrids derived from native North American Vitis species that are used to provide resistance against phylloxera and soilborne problems. A representative group of rootstock accessions and cultivars of the Vitis species commonly used in rootstock breeding (V. vinifera, V. berlandieri, V. riparia, and V. rupestris) and conserved in the largest European germplasm banks of Vitis were analyzed using sequence tagged microsatellite sites (STMS) and amplified fragment length polymorphism (AFLP) markers. The STMS analysis allowed assigning a microsatellite genotype to most of root-stock cultivars, although it revealed numerous misclassified accessions in the studied collections. Genetic similarity among the different genotypes was analyzed using AFLP, which provided information on the genetic relationships within and between hybrid groups.

Key words: AFLP, microsatellite, misclassified, Vitis






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Copyright © 2007 by the American Society for Enology and Viticulture.