HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Downey, M. O. Articles by Krstic, M. P. Search for Related Content PubMed Articles by Downey, M. O. Articles by Krstic, M. P. Agricola Articles by Downey, M. O. Articles by Krstic, M. P.

Development of a Stable Extract for Anthocyanins and Flavonols from Grape Skin

¹ Primary Industries Research Victoria, P.O. Box 905, Mildura, VIC 3502, Australia.

^* Corresponding author (email: mark.downey{at}dpi.vic.gov.au; fax: +61 3 50514523)

The lability of anthocyanins and flavonols extracted from grape (Vitis vinifera L.) skin with commonly used extraction solvents was examined. Results indicate that both anthocyanins and flavonol glycosides are unstable in even mildly acidic extraction solvents and highly labile in 1% hydrochloric acid in methanol, the most commonly used extraction solvent. While lability of anthocyanins has previously been reported, this is the first report of flavonol glycoside stability in these solvents. As a result of these observations, an extraction protocol was developed that maximized anthocyanin (malvidin-3-O-glucoside, malvidin-3-O-acetylglucoside, and malvidin-3-O-p-coumaroylglucoside) and flavonol (quercetin-3-O-glucoside and quercetin-3-O-glucuronide) extraction while minimizing degradation in the time between extraction and HPLC analysis. A range of acidified methanol solvents including hydrochloric, formic, acetic, citric, and maleic acids were tested together with acidulated ethanol and several methanol water mixtures ranging from 0% to 100% methanol. The most efficacious solvent system was 50% aqueous methanol, which had the advantage of incorporating both high anthocyanin and flavonol extractability and subsequent stability prior to HPLC analysis.

Key words: anthocyanin, flavonol, malvidin-glucoside, quercetin-glucoside, stability, extraction, solvent

This article has been cited by other articles:

				S. Czemmel, R. Stracke, B. Weisshaar, N. Cordon, N. N. Harris, A. R. Walker, S. P. Robinson, and J. Bogs The Grapevine R2R3-MYB Transcription Factor VvMYBF1 Regulates Flavonol Synthesis in Developing Grape Berries Plant Physiology, November 1, 2009; 151(3): 1513 - 1530. [Abstract] [Full Text] [PDF]