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Am. J. Enol. Vitic. 59:3:248-254 (2008)
Copyright © 2008 by the American Society for Enology and Viticulture.
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Use of Genetic Markers to Assess Pedigrees of Grape Cultivars and Breeding Program Selections

John Bautista1, Gerald S. Dangl2, Judy Yang1, Bruce Reisch3 and Ed Stover4,*

1 Laboratory assistant, 4 Curator & research leader, USDA, ARS National Clonal Germplasm Repository, University of California, Davis, CA 95616; 2 Manager, Plant Identification Lab, Foundation Plant Services, University of California, Davis, CA 95616; and 3 Professor, Department of Horticultural Sciences, Cornell University, New York State Agricultural Experiment Station, Geneva, New York 14456.

Corresponding author (email: ed.stover{at}ars.usda.gov; fax: 530 752-5974)

In a plant breeding program, an accurate understanding of pedigrees provides useful guidance for future hybridizations. However, plant breeder records occasionally contain errors which may mislead future breeding efforts, and there is considerable value in independently testing reported pedigrees. In this project, SSR markers were used to confirm or correct pedigrees for grape varieties from the Cornell breeding program and a few key varieties used as parents. As expected, most (20 of 24) reported pedigrees were confirmed. Recognizing a heritable null allele from Ontario, at VVMD25, was necessary for parent progeny analysis of several varieties. Suffolk Red and Glenora were shown to be progeny of Black Kishmish and not Black Monukka. The advanced selection NY63.0970.07 is a hybrid of Pinot noir, not Gamay, x Chancellor. Vignoles (Ravat 51) is not descended from the reported parentage of Seibel 6905 x Pinot noir.

Key words: crop improvement, microsatellite, null allele, SSR, Vitis






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Copyright © 2008 by the American Society for Enology and Viticulture.