AJEV
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Enol. Vitic. 60:1:104-109 (2009)
Copyright © 2009 by the American Society for Enology and Viticulture.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wegscheider, E.
Right arrow Articles by Forneck, A.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Wegscheider, E.
Right arrow Articles by Forneck, A.
Agricola
Right arrow Articles by Wegscheider, E.
Right arrow Articles by Forneck, A.

Research Note

Clonal Variation in Pinot noir Revealed by S-SAP Involving Universal Retrotransposon-Based Sequences

Eva Wegscheider1, Andrej Benjak2 and Astrid Forneck2,*

1 Master of science and 2 Doctor, University of Natural Resources and Applied Biotechnology, Institute of Horticulture and Viticulture, Peter Jordan Str. 82, A-1190 Vienna, Austria.

* Corresponding author (email: astrid.forneck{at}boku.ac.at)

A modified S-SAP (sequence-specific amplified polymorphism) method with universal primers for retrotransposons was used to study Vitis vinifera cv. Pinot noir. Five Pinot noir clones (20Gm, 1–44Gm, 18Gm, 20–13Gm, 1–84Gm) were analyzed by 30 S-SAP primer combinations employing five MseI- and six universal retrotransposon primers. Altogether 670 markers were generated, revealing 4.8% clonal variation and four out of five Pinot noir clones could be differentiated. This S-SAP method provides an efficient tool to randomly screen for polymorphisms produced through retrotransposition processes in the Vitis genome.

Key words: retrotransposon, Vitis, clonal variation, S-SAP






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2009 by the American Society for Enology and Viticulture.