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Research Note |
1 Misión Biologica de Galicia–CSIC, PO Box 28, Pontevedra, Spain; and 2 Instituto de la Vid y el Vino de Castilla La Mancha, Ctra. Toledo, Albacete s/n, 13700 Tomelloso, Ciudad Real, Spain.
Acknowledgments: M. Vilanova and M. de La Fuente are grateful to Consellería de Innovación e Industria from Xunta de Galicia for financing. The authors thank DO Ribeira Sacra, especially Ana López Pardo, for the localization of minority cultivars and thank J.M. Martínez-Zapater and J.R. Vidal for their assistance.
Twenty-five cultivars grown in Galicia (northwest Spain) and other six international cultivars have been analyzed in order to evaluate the genetic variability and cultivar relationships among them. Statistical analysis of 23 different genotypes found from six microsatellite markers (VVS2, VVMD5, VVMD7, VVMD27, ZAG62, and ZAG79) has shown three different groups. Several new synonymies were found among the minority cultivars analyzed. All the genotypes were compared to other databases and five more synonymies were found. A dendrogram shows the genetic similarities among the accessions and establishes the most probable relationship among them. Results also confirm, complement, and add new information to previous studies that have suggested the use of the variability in the phenolic compounds as a tool to identify phylogenetic relationships among these cultivars.
Key words: SSR, minority cultivars, Galicia, Spain
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