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Parentage Analysis of Freedom Rootstock

¹ USDA-ARS Grape Genetics Research Unit, 630 W. North St., Geneva, NY 14456, and ² USDA-ARS San Joaquin Valley Agricultural Sciences Center, 9611 S. Riverbend Ave., Parlier, CA 93648.

Acknowledgments: This research was financially supported by the USDA-ARS (projects 1910-21220-004-00 and 1910-21220-003-00).

The authors thank Warren Lamboy for initial assistance with computational analysis; Kathleen Deys, Rick Emershad, Hong Lin, and Parminder Sahota for technical assistance; Dave Matthews and Gayle Volk for comments and suggestions on the manuscript; and two anonymous reviewers for valuable comments.

^* Corresponding author (email: amanda.garris{at}ars.usda.gov)

The rootstock Freedom is the offspring of two open-pollinated parents (1613-59 and Dog Ridge 5), sources of root-knot nematode resistance. As phylloxera has increased in importance in vineyards, the risk of Vitis vinifera-derived phylloxera susceptibility from the unknown pollen donors is a concern. To clarify the pedigree of Freedom, genetic profiles of 31 potential progenitors were analyzed with 30 microsatellite markers. We confirm the reported parent (1613-59) and grandparent (1613C) of Freedom and identify 3306C (V. riparia x V. rupestris) as the pollen parent of 1613-59. We were unable to identify the pollen parent of Dog Ridge 5.

Key words: grapevine, microsatellite, rootstock, parentage