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Am. J. Enol. Vitic. 60:3:362-367 (2009)
Copyright © 2009 by the American Society for Enology and Viticulture.
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Research Note

Low Expression of Flavonoid 3',5'-Hydroxylase (F3',5'H) Associated with Cyanidin-based Anthocyanins in Grape Leaf

Hironori Kobayashi1,2,*, Shunji Suzuki1, Fumiko Tanzawa2 and Tsutomu Takayanagi1

1 Laboratory of Fruit Genetic Engineering, The Institute of Enology and Viticulture, University of Yamanashi, 1-13-1 Kofu, Yamanashi 400-0005, Japan, and 2 Laboratory of Enology & Viticulture, Mercian Corporation, 4-9-1 Jyonan, Fujisawa, Kanagawa 251-0057, Japan.

* Corresponding author (email: kobayashi-hr{at}mercian.co.jp; tel.: +81-55-220- 8394; fax: +81-55-220-8768)

The aim of this study was to clarify anthocyanin composition and transcription profiles of anthocyanin-synthesis-related genes in grape (Vitis vinifera L.) leaf during development. Berry skin accumulated both cyanidin-based anthocyanins and delphinidin-based anthocyanins during development and showed up-regulation of flavonoid 3',5'-hydroxylase gene (F3',5'H) expression. Grape leaf, in contrast, accumulated a large amount of cyanidin-based anthocyanins during development. This result was supported by the low expression of F3',5'H in developing grape leaf. The low expression of F3',5'H and the low level of delphinidin-based anthocyanins in grape leaf were consistent in green-yellow-, purple-, and black-colored skin cultivars. Flavonoid 3'-hydroxylase gene (F3'H) expression increased in grape leaf during development. Since F3',5'H regulates the synthesis of delphinidin-based anthocyanins and F3'H regulates the synthesis of cyanidin-based anthocyanins in plants, it is possible that grape leaf accumulates large amount of cyanidin-based anthocyanins through the cyanidin synthesis pathway regulated by F3'H.

Key words: flavonoid 3',5'-hydroxylase gene, flavonoid 3'-hydroxylase gene, cyanidin-based anthocyanin, delphinidin-based anthocyanin, grape






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Copyright © 2009 by the American Society for Enology and Viticulture.