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1 Instituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentario (IMIDRA), Finca El Encín, Carretera A-2, PK 38,200, 28800 Alcalá de Henares, Spain
2 present address, Instituto de Ciencias de la Vid y del Vino (ICVV), Complejo Científico Tecnológico, C/Madre de Dios 5, 26006 Logroño, Spain.
Acknowledgments: This study was made possible with the funding from the RF99-009 project (INIA, Agriculture Ministry of Spain) and of the GrapeGen project (joint venture between Genome Canada and Genoma España). A.M. Vargas was funded by a predoctoral fellowship from IMIDRA.
Information on the origin of elite table-grape cultivars is essential for breeding programs. A parentage study on table-grape accessions based on analysis of 25 nuclear and five chloroplast microsatellite loci was carried out. The pedigree of 50 cultivars was analyzed, including morphology studies when necessary. The chlorotypes clarify the direction of the crosses in the majority of cases, while likelihood ratios indicate high reliability of the proposed crosses. Nineteen new pedigrees are proposed for varieties from highly diverse geographical origins, such as Circé, Imperial Roja, Misket Vratchanskii, Mistress Hall, and Pizzutello Nero. Mistakes were also detected in previously described pedigrees for 13 cultivars, including Delizia di Vaprio and Madeleine Angevine. Alternative parents were identified for these 13 varieties. For another 18 varieties the previously proposed pedigree was confirmed. The cultivars Muscat of Alexandria, Afus Ali, Muscat Hamburg, and Chasselas were the most frequent parents of these 50 varieties.
Key words: chlorotype, genotyping, microsatellite, parentage analysis, Vitis vinifera L
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