Abstract
A simple modification, the addition of SO2 to the mobile phases, to an existing HPLC method for the analysis of unbleached polymeric pigments in red wines using a silica-based polymeric column is described for the first time. The samples included wines made from Pinot noir, Merlot, Cabernet Sauvignon, Cabernet franc, Sangiovese, Cagnulari, and Cannonau grapes, mostly from the 2001 season, which covered a range of wine color from 0.1 to 10 absorbance units at 520 nm. Results were compared to the level of SO2-resistant polymeric pigments as measured by an existing spectrophotometric assay (R2 = 0.992). In these wines, the ratio of the color of the bleached polymeric pigments to that of the unbleached polymers averaged 0.19 with a coefficient of variation of 34%. The SO2-resistant polymeric pigments estimated by this HPLC method are also in good agreement with those quantified by the protein precipitation spectrophotometric method of Harbertson and Adams (R2 = 0.950).
- Received May 2005.
- Revision received August 2007.
- Copyright © 2007 by the American Society for Enology and Viticulture
Sign in for ASEV members
ASEV Members, please sign in at ASEV to access the journal online.
Sign in for Institutional and Non-member Subscribers
Log in using your username and password
Pay Per Article - You may access this article (from the computer you are currently using) for 2 day for US$10.00
Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.