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Supplemental Data
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Supplemental Table 1
Composition of model media for MS300 (Rossouw and Bauer 2009) and CDGJM (Henschke and Jiranek 1993). (Assimilable nitrogen was calculated as total nitrogen content excluding that from proline.)
Supplemental Table 2
Aroma compounds formed by Saccharomyces cerevisiae with odor activity values greater than 1.
Supplemental Figure 1
Fermentation kinetics of strains isoC9D and iso-C9DΔLEU+pGP564 grown in CDGJM lacking leucine. Fermentations were conducted in deep-well (∼2 mL) plates and are reported as averages for each time point (n = 40).
Supplemental Figure 2
Fermentation curve for replicate 1, replicate 2 of strain iso-C9DΔLEU+pGP564, and the uninoculated control, as measured by refractometry in Brix.
Supplemental Figure 3
A simplified version of the pathways involved in the production of the aroma compounds linked to leucine biosynthesis (Baichwal et al. 1983, Bollon 1974, González et al. 2000, Guymon et al. 1961, Hazelwood et al. 2008, Ingraham et al. 1961).
- Supplemental Data -