@article {Buyukkileciajev.2018.18068, author = {Ceylan Buyukkileci and Aysem Batur and Ali Oguz Buyukkileci and Haluk Hamamci}, title = {Temperature and Glycerol Formation: A Proposal to Explain the Causal Relationship Based on Glycolytic Enzyme Activities}, elocation-id = {ajev.2018.18068}, year = {2018}, doi = {10.5344/ajev.2018.18068}, publisher = {American Journal of Enology and Viticulture}, abstract = {Most yeast strains produce glycerol in larger quantities when cultivated at higher temperatures. This is probably the reason why red wines contain higher amounts of glycerol than white wines. In this work, we tried a kinetic and thermodynamic approach to suggest a mechanistic reason for this phenomenon. A glycolytic model consisting of the kinetics of the individual enzymes constituting it was the starting point. The temperature and ethanol effects on the apparent kinetics of individual enzymes were determined and were incorporated into the model. The Arrhenius equation energy of activation was determined for each enzyme and it was found that the enzymes in the upper part of the glycolytic pathway were more dependent on the temperature. The model improved with these changes could qualitatively simulate the ethanol and glycerol production curves and that more glycerol is produced at higher temperatures. Here we propose that the differences in the temperature dependence of the enzymes around the glycerol branch are the reason for glycerol accumulation at higher temperatures.}, issn = {0002-9254}, URL = {https://www.ajevonline.org/content/early/2018/12/13/ajev.2018.18068}, eprint = {https://www.ajevonline.org/content/early/2018/12/13/ajev.2018.18068.full.pdf}, journal = {American Journal of Enology and Viticulture} }