PT - JOURNAL ARTICLE AU - Luciano Cinquanta AU - Donatella Albanese AU - Filippo De Curtis AU - Francesca Malvano AU - Alessio Crescitelli AU - Marisa Di Matteo TI - Rapid Assessment of Gray Mold (<em>Botrytis cinerea</em>) Infection in Grapes Using Biosensors System AID - 10.5344/ajev.2015.15029 DP - 2015 Aug 06 TA - American Journal of Enology and Viticulture PG - ajev.2015.15029 4099 - http://www.ajevonline.org/content/early/2015/08/04/ajev.2015.15029.short 4100 - http://www.ajevonline.org/content/early/2015/08/04/ajev.2015.15029.full AB - Botrytis cinerea is responsible for the gray mold disease, which causes considerable economic losses for winemakers. Its evaluation in wine grapes is commonly performed through visual estimation, which was demonstrated to be prone to assessor bias. Rapid and simple enzymatic carbon screen printed amperometric biosensors were here used to evaluate gluconic acid and glycerol content on wine grapes at different B. cinerea infection degrees. The lower concentrations measurable by screen-printed amperometric biosensors were 3 mg/L for gluconic acid (corresponding to an infection degree lower than 1%) and 35 mg/L for glycerol; the response times with a flow rate of 0.5 mL/min were in a range of 0.5 to 2 min in the linear ranges. This study demonstrates the effectiveness of the biosensors for rapid analysis of gluconic acid and glycerol in grapes, confirming their high correlation with B. cinerea degree of infection (R2 = 0.98). Thus, the biosensor developed to measure gluconic acid in grapes (or must), was more precise, and gave a faster response than methods that currently exist allowing the percentage of infection of grape berries by B. cinerea to be evaluated.