RT Journal Article SR Electronic T1 Rapid Assessment of Gray Mold (Botrytis cinerea) Infection in Grapes Using Biosensors System JF American Journal of Enology and Viticulture JO Am. J. Enol. Vitic. FD American Society for Enology and Viticulture SP ajev.2015.15029 DO 10.5344/ajev.2015.15029 A1 Luciano Cinquanta A1 Donatella Albanese A1 Filippo De Curtis A1 Francesca Malvano A1 Alessio Crescitelli A1 Marisa Di Matteo YR 2015 UL http://www.ajevonline.org/content/early/2015/08/04/ajev.2015.15029.abstract AB Botrytis cinerea is responsible for the gray mold disease, which causes considerable economic losses for winemakers. Its evaluation in wine grapes is commonly performed through visual estimation, which was demonstrated to be prone to assessor bias. Rapid and simple enzymatic carbon screen printed amperometric biosensors were here used to evaluate gluconic acid and glycerol content on wine grapes at different B. cinerea infection degrees. The lower concentrations measurable by screen-printed amperometric biosensors were 3 mg/L for gluconic acid (corresponding to an infection degree lower than 1%) and 35 mg/L for glycerol; the response times with a flow rate of 0.5 mL/min were in a range of 0.5 to 2 min in the linear ranges. This study demonstrates the effectiveness of the biosensors for rapid analysis of gluconic acid and glycerol in grapes, confirming their high correlation with B. cinerea degree of infection (R2 = 0.98). Thus, the biosensor developed to measure gluconic acid in grapes (or must), was more precise, and gave a faster response than methods that currently exist allowing the percentage of infection of grape berries by B. cinerea to be evaluated.