RT Journal Article
SR Electronic
T1 Regulation of Anthocyanin Biosynthesis in Tissues of a Teinturier Grape Cultivar under Sunlight Exclusion
JF American Journal of Enology and Viticulture
JO Am. J. Enol. Vitic.
FD American Society for Enology and Viticulture
SP ajev.2014.14029
DO 10.5344/ajev.2014.14029
A1 Le Guan
A1 Ji-Hu Li
A1 Pei-Ge Fan
A1 Shao-Hua Li
A1 Jin-Bao Fang
A1 Zhan-Wu Dai
A1 Serge Delrot
A1 Li-Jun Wang
A1 Ben-Hong Wu
YR 2014
UL http://www.ajevonline.org/content/early/2014/06/13/ajev.2014.14029.abstract
AB Yan-73 (Vitis Vinifera) is a teinturier grape cultivar, which accumulates anthocyanins in skin, pulp, pedicels and rachis. The effects of sunlight on anthocyanin biosynthesis and regulation in various tissues of this teinturier cultivar were investigated. Light transmission measurements showed that covering with opaque boxes substantially reduced light intensity around clusters; ≤ 0.25% of incident light reached the berry skin and <0.05% reached to the pulp. The berry pulp naturally experiences increasing sunlight-exclusion by berry skin during ripening. Sunlight exclusion reduced and delayed anthocyanin biosynthesis in berry skin and pulp during berry development, while both tissues nevertheless accumulated enough anthocyanins to turn dark red. No strong reduction of anthocyanin concentration was observed in pedicels or rachis. Sunlight exclusion decreased transcript abundance of VvUFGT, VvMybA1, VvMybA2, and VvMyc1 in both berry skin and pulp, and additionally decreased transcript abundance of VvMycA1 in berry pulp. Sunlight exclusion differently influenced the anthocyanin components: decreased the relative proportion of 3′,4′,5′-hydroxylated anthocyanins and increased that of 3′,4′-hydroxylated anthocyanins in berry skin and pulp, which corresponded to the change in the ratio of VvF3′H to VvF3′5′H. These results gained an insight of anthocyanin biosynthesis in various grape tissues in absence of sunlight.