RT Journal Article SR Electronic T1 Genotyping of Grapevine Varieties from Garfagnana (Northern Tuscany): Evidence of a Historical Center of Diversity JF American Journal of Enology and Viticulture JO Am. J. Enol. Vitic. FD American Society for Enology and Viticulture SP ajev.2015.15046 DO 10.5344/ajev.2015.15046 A1 Claudio D’Onofrio A1 Catia Fausto A1 Fabiola Matarese A1 Alberto Materazzi A1 Giancarlo Scalabrelli A1 Fabiana Fiorani A1 Ivo Poli YR 2015 UL http://www.ajevonline.org/content/early/2015/10/29/ajev.2015.15046.abstract AB The aim of this research was to recover, characterize and increase the value of local grapevine varieties from Garfagnana, a mountain area situated in the north of Tuscany (central Italy). A total of 130 accessions (vines) were identified in old vineyards, characterized by OIV morphological-phenological and productive parameters, and genotyped using 14 microsatellite loci. Microsatellite analysis led to the identification of 50 genotypes. Some matched Tuscan genotypes, others corresponded to varieties cultivated in other Italian and European regions, and 18 appeared to be genotypes currently identified only in Garfagnana and presumably autochthonous of this area. The cluster and similarity analysis on both microsatellites and morphology showed a clear grouping of the majority of the autochthonous genotypes from Garfagnana. The parentage analysis also revealed that Garfagnana autochthonous genotypes are highly first degree related among them, suggesting that Garfagnana is a distinct historical centre of diversity of cultivated varieties. All varieties have been uploaded in the Italian Vitis Database (www.vitisdb.it).