%0 Journal Article %A Bruce M. Freeman %A Carlos J. Muller %A Richard E. Kepner %A A. Dinsmoor Webb %T Some Products of the Metabolism of Ethyl 4-Oxobutanoate by Saccharomyces Fermentati in the Film Form on the Surface of Simulated Flor Sherry %D 1977 %R 10.5344/ajev.1977.28.2.119 %J American Journal of Enology and Viticulture %P 119-122 %V 28 %N 2 %X Volatile metabolites of ethyl 4-oxobutanoate were isolated from cultures of Saccharomyces fermentati growing in the film form on the surface of media synthesized to resemble flor sherry but contain ethanol as the only volatile carbon containing compound. Metabolites were isolated by distillation followed by extraction and evaporation of the extracting solvent. Volatiles were identified by gas-chromatographic retention times, and IR (infrared), NMR (nuclear magnetic resonance) and MS (mass) spectrometry. Saccharomyces fermentati growing as a film on the surface of a 13% v/v ethanol, pH 3.4 medium converts ethyl 4-oxobutanoate into diethyl succinate, ethyl 4-hydroxybutanoate and 4-hydroxybutanoic acid lactone. 2-Phenethanol and 2- and 3-methylbutanol, and trace amounts of other unidentified compounds were formed. %U https://www.ajevonline.org/content/ajev/28/2/119.full.pdf