PT  - JOURNAL ARTICLE
AU  - Bruce M. Freeman
AU  - Carlos J. Muller
AU  - Richard E. Kepner
AU  - A. Dinsmoor Webb
TI  - Some Products of the Metabolism of Ethyl 4-Oxobutanoate by Saccharomyces Fermentati in the Film Form on the Surface of Simulated Flor Sherry
AID  - 10.5344/ajev.1977.28.2.119
DP  - 1977 Jan 01
TA  - American Journal of Enology and Viticulture
PG  - 119--122
VI  - 28
IP  - 2
4099  - http://www.ajevonline.org/content/28/2/119.short
4100  - http://www.ajevonline.org/content/28/2/119.full
SO  - Am J Enol Vitic.1977 Jan 01; 28
AB  - Volatile metabolites of ethyl 4-oxobutanoate were isolated from cultures of Saccharomyces fermentati growing in the film form on the surface of media synthesized to resemble flor sherry but contain ethanol as the only volatile carbon containing compound. Metabolites were isolated by distillation followed by extraction and evaporation of the extracting solvent. Volatiles were identified by gas-chromatographic retention times, and IR (infrared), NMR (nuclear magnetic resonance) and MS (mass) spectrometry. Saccharomyces fermentati growing as a film on the surface of a 13% v/v ethanol, pH 3.4 medium converts ethyl 4-oxobutanoate into diethyl succinate, ethyl 4-hydroxybutanoate and 4-hydroxybutanoic acid lactone. 2-Phenethanol and 2- and 3-methylbutanol, and trace amounts of other unidentified compounds were formed.