PT  - JOURNAL ARTICLE
AU  - Wade H. Wolfe
TI  - Identification of Grape Varieties by Isozyme Banding Patterns
AID  - 10.5344/ajev.1976.27.2.68
DP  - 1976 Jan 01
TA  - American Journal of Enology and Viticulture
PG  - 68--73
VI  - 27
IP  - 2
4099  - http://www.ajevonline.org/content/27/2/68.short
4100  - http://www.ajevonline.org/content/27/2/68.full
SO  - Am J Enol Vitic.1976 Jan 01; 27
AB  - Enzyme banding patterns for over 60 varieties of wine and table grapes were determined by gel electrophoresis. Enzymes were extracted from ripe berries of each variety and separated by electrophoresis in a starch gel. Enzyme bands were detected by developing the gels in a buffered solution that produced an insoluble dye at the site of enzyme activity. The varieties were assayed for leucine aminopeptidase, indophenol oxidase, acid phosphatase, catechol oxidase, alcohol dehydrogenase, esterase, and peroxidase. The first four enzymes listed were found the most useful for distinguishing varieties.