TY - JOUR T1 - Identification of Grape Varieties by Isozyme Banding Patterns JF - American Journal of Enology and Viticulture JO - Am J Enol Vitic. SP - 68 LP - 73 DO - 10.5344/ajev.1976.27.2.68 VL - 27 IS - 2 AU - Wade H. Wolfe Y1 - 1976/01/01 UR - http://www.ajevonline.org/content/27/2/68.abstract N2 - Enzyme banding patterns for over 60 varieties of wine and table grapes were determined by gel electrophoresis. Enzymes were extracted from ripe berries of each variety and separated by electrophoresis in a starch gel. Enzyme bands were detected by developing the gels in a buffered solution that produced an insoluble dye at the site of enzyme activity. The varieties were assayed for leucine aminopeptidase, indophenol oxidase, acid phosphatase, catechol oxidase, alcohol dehydrogenase, esterase, and peroxidase. The first four enzymes listed were found the most useful for distinguishing varieties. ER -