PT  - JOURNAL ARTICLE
AU  - Andrew H. Kuniyuki
AU  - Craig Rous
AU  - Janet L. Sanderson
TI  - Enzyme-Linked Immunosorbent Assay (ELISA) Detection of <em>Brettanomyces</em> Contaminants in Wine Production
AID  - 10.5344/ajev.1984.35.3.143
DP  - 1984 Jan 01
TA  - American Journal of Enology and Viticulture
PG  - 143--145
VI  - 35
IP  - 3
4099  - http://www.ajevonline.org/content/35/3/143.short
4100  - http://www.ajevonline.org/content/35/3/143.full
SO  - Am J Enol Vitic.1984 Jan 01; 35
AB  - The application of hybridoma-derived antibodies to the detection of Brettanomyces contaminants in wine production has increased the sensitivity and speed of detection. Utilizing the antibodies in the enzyme-linked immunosorbent assay (ELISA), 91.5 x 10-12 g of protein can be detected with a Titertek Multiskan MC having an absorbance threshold being the equivalent of 34 cells per mL. A double blind test of direct wine samples proved the sensitivity of the analysis in detecting contaminated wine.