TY - JOUR T1 - Enzyme-Linked Immunosorbent Assay (ELISA) Detection of <em>Brettanomyces</em> Contaminants in Wine Production JF - American Journal of Enology and Viticulture JO - Am J Enol Vitic. SP - 143 LP - 145 DO - 10.5344/ajev.1984.35.3.143 VL - 35 IS - 3 AU - Andrew H. Kuniyuki AU - Craig Rous AU - Janet L. Sanderson Y1 - 1984/01/01 UR - http://www.ajevonline.org/content/35/3/143.abstract N2 - The application of hybridoma-derived antibodies to the detection of Brettanomyces contaminants in wine production has increased the sensitivity and speed of detection. Utilizing the antibodies in the enzyme-linked immunosorbent assay (ELISA), 91.5 x 10-12 g of protein can be detected with a Titertek Multiskan MC having an absorbance threshold being the equivalent of 34 cells per mL. A double blind test of direct wine samples proved the sensitivity of the analysis in detecting contaminated wine. ER -