RT Journal Article
SR Electronic
T1 The Origin of Embryos and Plantlets from Cultured Anthers of Hybrid Grapevines
JF American Journal of Enology and Viticulture
JO Am J Enol Vitic.
FD American Society for Enology and Viticulture
SP 108
OP 113
DO 10.5344/ajev.1983.34.2.108
VO 34
IS 2
A1 K. Rajasekaran
A1 Michael G. Mullins
YR 1983
UL http://www.ajevonline.org/content/34/2/108.abstract
AB Callus derived from cultured anthers of Gloryvine (Vitis vinifera L. x V. rupestris Scheele) contained both haploid (n=19) and diploid (2n=38) cells, but all the plantlets produced in vitro were diploid. The presence of haploid cells in callus derived from anthers was further indicated by the occurrence of nuclei with the 1C-DNA level. Haploid cells were not found in callus from internode explants. Multinucleate pollen grains were observed, but there was strong anatomical evidence that the anther-callus originated primarily from somatic cells of the anthers wall and the connective. There was considerable variation among anther-derived plants in leaf morphology. Progeny of selfed anther-derived plants exhibited segregation for leaf characters indicating heterozygosity and somatic origin. Isoenzyme patterns for indophenol oxidase, leucine amino peptidase and catechol oxidase in anther-derived plants and in the original genotype from which the cultures were established were indistinguishable.