RT Journal Article
SR Electronic
T1 Ethanol Determination in Wine with an Immobilized Enzyme Electrode
JF American Journal of Enology and Viticulture
JO Am J Enol Vitic.
FD American Society for Enology and Viticulture
SP 173
OP 175
DO 10.5344/ajev.1983.34.3.173
VO 34
IS 3
A1 Marc Mason
YR 1983
UL http://www.ajevonline.org/content/34/3/173.abstract
AB The purpose of this work was to demonstrate the accuracy and precision of a new method for ethanol determination. This method utilizes a thin microporous membrane containing immobilized alcohol oxidase (E.C. 1.1.3.13) which is mounted on an amperometric electrode. When ethanol enters the membrane it is rapidly oxidized producing hydrogen peroxide and acetaldehyde. The hydrogen peroxide is oxidized at the electrode giving rise to a signal current directly proportional to hydrogen peroxide concentration, and hence to ethanol concentration. The coefficient of variation for six analyses of eight wine samples was better than 1.0%, and agreement with A.O.A.C. specific gravity method was excellent.