TY - JOUR T1 - Lipoxygenase Activity in Grapes (cv. Macabeo). I. Evidence and Partial Purification JF - American Journal of Enology and Viticulture JO - Am J Enol Vitic. SP - 316 LP - 318 DO - 10.5344/ajev.1985.36.4.316 VL - 36 IS - 4 AU - R. Zamora AU - J. I. Maynar AU - J. L. Mesías Y1 - 1985/01/01 UR - http://www.ajevonline.org/content/36/4/316.abstract N2 - Lipoxygenase activity in grapes (cv. Macabeo) was evidenced as an increase in absorbance at 234 nm. Lipoxygenase was isolated by ammonium sulfate fractionation, hydrophobic chromatography. Elution of the enzyme from the resin was performed by a linear gradient of (NH4)2SO4 and ethylene glycol. The lipoxygenase activity was higher in partially ripe than in fully ripe grapes. ER -