TY - JOUR T1 - The Occurrence of <em>Agrobacterium tumefaciens</em> in Grapevine-Propagating Material and a Simplified Indexing System JF - American Journal of Enology and Viticulture JO - Am J Enol Vitic. SP - 189 LP - 193 DO - 10.5344/ajev.1987.38.3.189 VL - 38 IS - 3 AU - R. N. Goodman AU - D. Butrov AU - F. Tarbah Y1 - 1987/01/01 UR - http://www.ajevonline.org/content/38/3/189.abstract N2 - Grapevine-propagating material from two vineyards and one nursery in Missouri and one California nursery carry Agrobacterium tumefaciens latently in their vascular systems. A rapid yet simple procedure for detection of A. tumefaciens in grapevine-propagating material was developed. The process depends upon displacement of vascular fluids by sterile water in a low-pressure chamber. Expelled fluid is placed on a selective medium that readily permits discrimination of the pathogen from contaminants. Plants examined three times without revealing the presence of any bacteria in their xylem fluids are regarded as free of pathogen. ER -