@article {Frayne281, author = {R. F. Frayne}, title = {Direct Analysis of the Major Organic Components in Grape Must and Wine Using High Performance Liquid Chromatography}, volume = {37}, number = {4}, pages = {281--287}, year = {1986}, doi = {10.5344/ajev.1986.37.4.281}, publisher = {American Journal of Enology and Viticulture}, abstract = {A high performance liquid chromatography (HPLC) method has been developed for the isocratic separation and quantification of the major organic acids, sugars, and alcohols in wine and grape must using a dual cation exchange column system without sample preparation. The sample is filtered through a 0.45-{\textmu}m membrane and injected, separated, and quantified in less than 40 minutes. Statistical evaluation of the HPLC method showed it to be highly reproducible, reliable, and stable. A comparison was made between the HPLC method and conventional volumetric, distillation, and enzymatic methods of analysis. The statistical analysis showed good agreement in accuracy and precision between the methods compared.}, issn = {0002-9254}, URL = {https://www.ajevonline.org/content/37/4/281}, eprint = {https://www.ajevonline.org/content/37/4/281.full.pdf}, journal = {American Journal of Enology and Viticulture} }