RT Journal Article SR Electronic T1 Extraction of Protein From Wine Lees JF American Journal of Enology and Viticulture JO Am J Enol Vitic. FD American Society for Enology and Viticulture SP 65 OP 66 DO 10.5344/ajev.1989.40.1.65 VO 40 IS 1 A1 Vito Sciancalepore YR 1989 UL http://www.ajevonline.org/content/40/1/65.abstract AB The extraction procedure and the effects of sodium hydroxide concentration, time, and temperature of extraction on protein extractability from wine lees were evaluated. An optimum was found for disintegrated wine lees after extraction for 30 minutes at 50°C with 0.8% sodium hydroxide at pH 12.5. The Kjeldahl protein obtained by acid precipitation (pH 3.5) of the alkaline extract represented 45% of total wine-lees protein.