@article {Bissell316, author = {Peter Bissell and Andrew Ewart and Wanlee Sangtippawan}, title = {Loading Concentrations for Tartaric and Malic Acid for Single Column HPLC Organic Acid Analysis}, volume = {40}, number = {4}, pages = {316--319}, year = {1989}, doi = {10.5344/ajev.1989.40.4.316}, publisher = {American Journal of Enology and Viticulture}, abstract = {The linearity of detection of tartaric and malic acids in grape juice was investigated for a single Aminex HPX 87H organic acid analysis column system. High levels of tartaric and malic acid resulted in a major drop in percent recovery. Using external standard calibration, based on peak height integration, the upper limits for column loading of grape juice samples were found to be 10 {\textmu}g for tartaric acid and 30 {\textmu}g for malic acid, above which recoveries dropped below 95\%. A similar result was found using a synthetic juice containing glucose, fructose, and the two acids. Aqueous solutions of acids were found to be linear up to 25 {\textmu}g tartaric acid and 130 {\textmu}g malic acid. The reason for the drop in recovery under high acid conditions was not identified. Routine grape juice analysis required dilution of 1:10 to 1:20 to ensure accurate recoveries.}, issn = {0002-9254}, URL = {https://www.ajevonline.org/content/40/4/316}, eprint = {https://www.ajevonline.org/content/40/4/316.full.pdf}, journal = {American Journal of Enology and Viticulture} }