PT  - JOURNAL ARTICLE
AU  - R. M. Marcé
AU  - M. Calull
AU  - F. Borrull
AU  - F. X. Rius
TI  - Determination of Major Carboxylic Acids in Wine by an Optimized HPLC Method with Linear Gradient Elution
AID  - 10.5344/ajev.1990.41.4.289
DP  - 1990 Jan 01
TA  - American Journal of Enology and Viticulture
PG  - 289--294
VI  - 41
IP  - 4
4099  - http://www.ajevonline.org/content/41/4/289.short
4100  - http://www.ajevonline.org/content/41/4/289.full
SO  - Am J Enol Vitic.1990 Jan 01; 41
AB  - An optimized method to determine carboxylic acids in wine based on pre-column derivatization with phenacyl bromide is reported. The determination of the different acids has been carried out using a linear gradient elution involving water and methanol. The method has been optimized by the Simplex method using a modification of CRF (chromatographic response factor) as the criterion for selectivity optimization. After validation, the method was applied to determine carboxylic acids in red and white wines and grape must of the Tarragona region.