RT Journal Article
SR Electronic
T1 Determination of Major Carboxylic Acids in Wine by an Optimized HPLC Method with Linear Gradient Elution
JF American Journal of Enology and Viticulture
JO Am J Enol Vitic.
FD American Society for Enology and Viticulture
SP 289
OP 294
DO 10.5344/ajev.1990.41.4.289
VO 41
IS 4
A1 R. M. Marcé
A1 M. Calull
A1 F. Borrull
A1 F. X. Rius
YR 1990
UL http://www.ajevonline.org/content/41/4/289.abstract
AB An optimized method to determine carboxylic acids in wine based on pre-column derivatization with phenacyl bromide is reported. The determination of the different acids has been carried out using a linear gradient elution involving water and methanol. The method has been optimized by the Simplex method using a modification of CRF (chromatographic response factor) as the criterion for selectivity optimization. After validation, the method was applied to determine carboxylic acids in red and white wines and grape must of the Tarragona region.