RT Journal Article
SR Electronic
T1 Double-Stranded RNA Isolated From Grapevines Affected by Rupestris Stem Pitting Disease
JF American Journal of Enology and Viticulture
JO Am J Enol Vitic.
FD American Society for Enology and Viticulture
SP 175
OP 179
DO 10.5344/ajev.1991.42.3.175
VO 42
IS 3
A1 M. H. Walter
A1 H. R. Cameron
YR 1991
UL http://www.ajevonline.org/content/42/3/175.abstract
AB A survey of healthy and rupestris stem pitting (SP) diseased grapevines has been made for the presence of double-stranded RNA (dsRNA). Commercially important Pinot noir and Pinot gris clones from Oregon State University (OSU) were selected on the basis of virus disease indexing results. Double-stranded RNA was extracted from leaves and dormant cane phloem of mature grapevines, purified by column chromatography, and characterized by RNase digestion, lithium chloride precipitation, acridine orange staining, and agarose gel electrophoresis. The single dsRNA species observed that was unique to SP diseased vines was approximately 5.5 x 106 daltons. All SP-diseased vines contained one or more species of dsRNA while virus-free tissues contained none. Antisera prepared against grapevine virus "A" and against grapevine "potyvirus-like virus" were used in preliminary enzyme linked immunosorbent assays and gel diffusion tests, but results were inconclusive. Uses of dsRNA analysis for detection of SP disease are discussed.