PT  - JOURNAL ARTICLE
AU  - Adib Rowhani
TI  - Use of F(ab')<sub>2</sub> Antibody Fragment in ELISA for Detection of Grapevine Viruses
AID  - 10.5344/ajev.1992.43.1.38
DP  - 1992 Jan 01
TA  - American Journal of Enology and Viticulture
PG  - 38--40
VI  - 43
IP  - 1
4099  - http://www.ajevonline.org/content/43/1/38.short
4100  - http://www.ajevonline.org/content/43/1/38.full
SO  - Am J Enol Vitic.1992 Jan 01; 43
AB  - A modification of the enzyme-linked immunosorbent assay using the F(ab')2 antibody fragment [F(ab')2 ELISA] was developed for the detection of grapevine fanleaf (GFLV) and grapevine leafroll (GLRV-type III) viruses. The protocol was sensitive and rapid. Sensitivity of the test was further enhanced by pre-mixing the detecting antibody (probe) and the enzyme-conjugated protein A (anti-probe). Due to the acidic nature of grapevine tissues, different extraction buffer compositions were also evaluated.