PT  - JOURNAL ARTICLE
AU  - Laurie Connell
AU  - Henrik Stender
AU  - Charles G. Edwards
TI  - Rapid Detection and Identification of <em>Brettanomyces</em> from Winery Air Samples Based on Peptide Nucleic Acid Analysis
AID  - 10.5344/ajev.2002.53.4.322
DP  - 2002 Jan 01
TA  - American Journal of Enology and Viticulture
PG  - 322--324
VI  - 53
IP  - 4
4099  - http://www.ajevonline.org/content/53/4/322.short
4100  - http://www.ajevonline.org/content/53/4/322.full
SO  - Am J Enol Vitic.2002 Jan 01; 53
AB  - A new filter-based chemiluminescent in situ hybridization method using peptide nucleic acid probes for rapid and simultaneous detection, identification, and enumeration of Brettanomyces from air samples was developed. The method also allows for detection of the yeast prior to visual appearance of large colonies. Using this technique, air samples from various locations within a winery (crush, tank, barrel, and bottling rooms) were analyzed. Brettanomyces was identified in samples taken from all four rooms, confirming that this spoilage yeast can be spread through a winery by air currents.