RT Journal Article SR Electronic T1 Effect of Shading and Nitrogen Source on Growth, Tissue Ammonium and Nitrate Status, and Inflorescence Necrosis in Pinot noir Grapevines JF American Journal of Enology and Viticulture JO Am J Enol Vitic. FD American Society for Enology and Viticulture SP 173 OP 180 DO 10.5344/ajev.1996.47.2.173 VO 47 IS 2 A1 Sanliang Gu A1 Porter B. Lombard A1 Steven F. Price YR 1996 UL http://www.ajevonline.org/content/47/2/173.abstract AB The effect of 60% shading and nitrogen (N) source on growth, tissue NH4+ and NO3- concentrations, and inflorescence necrosis (IN) was investigated in one- and two-year-old potted Pinot noir grapevines. New growth of the vines was significantly reduced by shading and NH4+ feeding resulted in less new growth compared to NO3- feeding. The shaded two-year-old vines developed 26.6% IN compared to only 0.6% on unshaded vines. No IN was induced by either NH4+ or NO3- feeding treatments. Shading increased tissue NH4+ concentration in most tissues of one-year-old vines with few exceptions and in laminas, petioles, and rachis of two-year-old vines. Two-year-old vines fed NH4+ and/or NO3- had a higher rachis, petiole, stem, and young root NH4+ concentration at post-anthesis, compared to the no N control. The NH4+-fed vines had a higher NH4+ concentration than that of NO3##-fed vines only in stems at post-anthesis and young roots pre-anthesis, anthesis, and post-anthesis. Tissue NO3- concentration of laminas, petioles, stems, and young roots was proportional to the amount of NO3--fed to the vines in the root medium. Elevated tissue NH4+ concentration in the rachis of shaded vines and increased incidence of IN suggest that IN is associated with elevated tissue NH4+ levels. NH4+ and/or NO3- in culture media is not a source of elevated NH4+ level for IN development.